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- Title
The Response of TLR3 and IL-1ß Genes Following Exposure to LPS, Poly (I:C), Zymosan in Culture of Gouramy (Osphronemus gouramy) Kidney Cells.
- Authors
Kusumawaty, Diah; Suhandono, Sony; Aryantha, I. Nyoman Pugeg; Pancoro, Adi
- Abstract
The aims of the present study are to isolate and characterise structure TLR3 and IL1ß gene and evaluate the potential and signaling mechanism following exposure of Polyinosinic: polycytidylic acid (poly (I:C)), Lipopolysaccharide (LPS) and Zymosan as antigens in gouramy (Osphronemus gouramy). Gouramy kidney cells were stimulated with LPS, Poly I:C and Zymosan. Following incubation at 28°C, relative expression levels of Toll Like Receptor 3 (TLR3) and interleukin-1ß (IL-1ß) were examined at one hour and six hour after treatment. A Real Time Polimerase Chain Reaction approach was utilised to search for the effects of Poly I:C, LPS, and Zymosan exposure to gouramy kidney cells between one hour to six hours after treatment, LPS in kidney cell increased expression of interleukin-1ß and downregulated expression of TLR3. Poly (I:C) which is an antigen that responds to antiviral, induces an increase in the transcription both of Toll like receptor 3 and IL-1ß. Zymosan in kidney cells increased expression of TLR3 but downregulated expression of IL1β. This study shows that TLR3 was activated not only by Poly I: C but also LPS and Zymosan. However, antigen Poly IC-induced labor IL-1ß and TLR3 expressed higher than the antigen LPS and Zymosan, so, this research showed a TLR3 response to Poly I:C is more dominant than Zymosan and LPS. TLR3 in gouramy kidney cell transcript was identified in response to poly I:C, Zymosan, and LPS. These results indicate that TLR3 in gouramy also plays a role for defense against bacterial infection and virus.
- Subjects
GOURAMI; ZYMOSAN; MECHANISM (Philosophy); LIPOPOLYSACCHARIDES; KIDNEY cell culture
- Publication
Pertanika Journal of Tropical Agricultural Science, 2018, Vol 41, Issue 3, p1479
- ISSN
1511-3701
- Publication type
Article