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- Title
Activation of amphibian oocytes by sperm extracts.
- Authors
F. Bonilla; M. T. Ajmat; G. S?nchez Toranzo; L. Zelaray?n; J. Oterino; M. I. B?hler
- Abstract
SummaryIn the fertilization of most animals, egg activation is accompanied by an increase in cytoplasmatic Ca2+; however, the mechanism through which the fertilizing sperm induce this phenomenon is still controversial. An increase in intracellular free Ca2+is required to trigger egg activation events, a process that includes cortical granule exocytosis, resumption and completion of meiosis and DNA replication, and culminates in the first mitotic cleavage. In this work, we investigated the effect of microinjection and incubation of different fractions of homologous sperm extract on the activation of Bufo arenarumoocytes matured in vitro. Two heat treatment-sensitive fractions obtained by chromatography were able to induce oocyte activation. The sperm fraction, which contained a 24 kDa protein, induced 90% activation when it was microinjected into the oocytes. Whilst the sperm fraction, which contained a 36 kDa protein, was able to induce about 70% activation only when it was applied on the oocyte surface.
- Subjects
OVUM; AMPHIBIANS; SPERMATOZOA; CYTOPLASM; CALCIUM channels; EMBRYOLOGY; EGG incubation; PROTEINS
- Publication
Zygote, 2008, Vol 16, Issue 4, p303
- ISSN
0967-1994
- Publication type
Article
- DOI
10.1017/S0967199408004814