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- Title
Genotyping of 11 human papillomaviruses by multiplex PCR with a GeXP analyzer.
- Authors
Yang, Meng-Jie; Luo, Le; Nie, Kai; Wang, Miao; Zhang, Chen; Li, Jin; Ma, Xue-jun
- Abstract
A new, rapid, and high-throughput method was developed for simultaneous detection of 11 human papillomavirus (HPV) genotypes including nine high-risk types (HPV16, 18, 31, 33, 35, 39, 52, 58, and 66) and two low-risk types (HPV6 and 11) in a single tube by multiplex PCR based on a GenomeLab Gene Expression Profiler (GeXP) analyzer (GeXP-PCR). Eleven sets of chimeric primers were used to initiate the PCR, and one pair of universal primers was used for the subsequent cycles of the PCR. The specificity of GeXP-PCR for each HPV type was examined with clinical samples of single type HPV infection tested previously. The sensitivity of GeXP-PCR was evaluated by performing the assay on serial 10-fold dilutions of cloned PCR products. The GeXP-PCR achieved a sensitivity of 100 copies when all of the 11 pre-mixed plasmids containing HPV targets were present. Analyses of 124 clinical specimens using the GeXP-PCR demonstrated that the GeXP-PCR assay had comparable sensitivity and specificity to those of reported multiple PCR assay and an increased detection of HPV 11 in samples with mixed infections. In conclusion, the GeXP-PCR is a fast, sensitive, and high throughput method for the detection of multiple HPV infections. J. Med. Virol. 84:957-963, 2012. © 2012 Wiley Periodicals, Inc.
- Publication
Journal of Medical Virology, 2012, Vol 84, Issue 6, p957
- ISSN
0146-6615
- Publication type
Article
- DOI
10.1002/jmv.23275