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- Title
MicroRNA 497 modulates interleukin 1 signalling via the MAPK/ERK pathway
- Authors
Zheng, Dongling; Radziszewska, Anna; Woo, Patricia
- Abstract
Abstract: The MAPK/ERK signalling pathway has been described to mediate IL-1 induction of target genes and is known to be regulated by microRNAs (miRNA). We describe a novel miRNA regulating the expression of the MEK1 gene and how it impacts IL-1 induced IL-6 transcription. miR-497 was predicted to target MEK1 3′UTR using bioinformatic tools. Transfection of miR-497 into HeLa cells inhibited MEK1 protein expression by 50%. In transient transfection experiments, the luciferase activity of a MEK1 3′UTR luciferase reporter construct was reduced in the presence of miR-497, and mutation of the predicted miR-497 binding site restored activity. miR-497 also decreased protein levels of RAF1 and ERK1 but not ERK2. Addition of miR-497 was further shown to inhibit IL-1 induced IL-6 gene transcription.
- Subjects
MICRORNA; INTERLEUKIN-1; MITOGEN-activated protein kinases; CELLULAR signal transduction; GENE expression; NON-coding RNA; POLYMERASE chain reaction; C-Jun N-terminal kinases
- Publication
FEBS Letters, 2012, Vol 586, Issue 23, p4165
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2012.10.014