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- Title
Analysis of solvent tolerance in Pseudomonas putida DOT-T1E based on its genome sequence and a collection of mutants
- Authors
Udaondo, Zulema; Duque, Estrella; Fernández, Matilde; Molina, Lázaro; Torre, Jesús de la; Bernal, Patricia; Niqui, José-Luis; Pini, Cecilia; Roca, Amalia; Matilla, Miguel A.; Antonia Molina-Henares, M.; Silva-Jiménez, Hortencia; Navarro-Avilés, Gloria; Busch, Andreas; Lacal, Jesús; Krell, Tino; Segura, Ana; Ramos, Juan-Luis
- Abstract
Abstract: Pseudomonas putida strains are prevalent in a variety of pristine and polluted environments. The genome of the solvent-tolerant P. putida strain DOT-T1E which thrives in the presence of high concentrations of monoaromatic hydrocarbons, contains a circular 6.3Mbp chromosome and a 133kbp plasmid. Omics information has been used to identify the genes and proteins involved in solvent tolerance in this bacterium. This strain uses a multifactorial response that involves fine-tuning of lipid fluidity, activation of a general stress-response system, enhanced energy generation, and induction of specific efflux pumps that extrude solvents to the medium. Local and global transcriptional regulators participate in a complex network of metabolic functions, acting as the decision makers in the response to solvents.
- Subjects
PSEUDOMONAS putida; AMINO acid sequence; GENETIC mutation; ORGANIC solvents; TRANSCRIPTION factors; GENETIC regulation; DECISION making; PLASMIDS
- Publication
FEBS Letters, 2012, Vol 586, Issue 18, p2932
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2012.07.031