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- Title
Structural mechanisms of RNA recognition: sequence-specific and non-specific RNA-binding proteins and the Cas9-RNA-DNA complex.
- Authors
Ban, Ting; Zhu, Jian-Kang; Melcher, Karsten; Xu, H.
- Abstract
RNA-binding proteins play crucial roles in RNA processing and function as regulators of gene expression. Recent studies have defined the structural basis for RNA recognition by diverse RNA-binding motifs. While many RNA-binding proteins recognize RNA sequence non-specifically by associating with 5′ or 3′ RNA ends, sequence-specific recognition by RNA-binding proteins is typically achieved by combining multiple modular domains to form complex binding surfaces. In this review, we present examples of structures from different classes of RNA-binding proteins, identify the mechanisms utilized by them to target specific RNAs, and describe structural principles of how protein-protein interactions affect RNA recognition specificity. We also highlight the structural mechanism of sequence-dependent and -independent interactions in the Cas9-RNA-DNA complex.
- Subjects
CHEMICAL structure; NUCLEOTIDE sequence; MOLECULAR recognition; DNA-binding proteins; GENE expression; PROTEIN structure; SURFACE chemistry
- Publication
Cellular & Molecular Life Sciences, 2015, Vol 72, Issue 6, p1045
- ISSN
1420-682X
- Publication type
Article
- DOI
10.1007/s00018-014-1779-9