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- Title
Granzyme B is recovered by natural killer cells via clathrin-dependent endocytosis.
- Authors
Pan Li; Guoying Zheng; Yan Yang; Chunguang Zhang; Ping Xiong; Yong Xu; Min Fang; Zheng Tan; Fang Zheng; Feili Gong
- Abstract
When they recognize a target cell, natural killer (NK) cells mount an attack to kill the target by exerting their cytotoxicity via the exocytosis of cytotoxic granules. Although the details of this process (which includes the movement of cytotoxic granules in the immune synapse and their fusion with the plasma membrane, releasing granzymes and perforin into the synaptic cleft) are relatively better understood, the post-exocytosis regulation of the process is still largely unknown. Here we show that a clathrin-dependent endocytosis stimulated by target cell occurs in NK92 cell line, which is closely correlated with granzyme B recovery. Inhibition of the endocytosis significantly attenuates the cytotoxicity of NK92 cells. The NK cell recovery of its released effector molecules, in turn, suggests that endocytosis may well play a key role in the post exocytosis regulation of immune cells.
- Subjects
KILLER cells; CELL lines; EXOCYTOSIS; CELL-mediated cytotoxicity; CELL membranes
- Publication
Cellular & Molecular Life Sciences, 2010, Vol 67, Issue 18, p3197
- ISSN
1420-682X
- Publication type
Article
- DOI
10.1007/s00018-010-0377-8