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- Title
Culturing embryonic cells from the parthenogenetic clonal marble crayfish (Marmorkrebs) Procambarus virginalis Lyko, 2017 (Decapoda: Astacidea: Cambaridae).
- Authors
Deleon, Heriberto; Garcia, Juan; Silva, Dionn Carlo; Quintanilla, Oscar; Faulkes, Zen; Thomas, John M
- Abstract
The parthenogenetic marbled crayfish, or Marmorkrebs (Procambarus virginalis Lyko 2017), is an emerging model organism. We describe a method to isolate cells from early-stage embryos and culture them in vitro. The identity of the cells was confirmed by sequencing the cytochrome c oxidase subunit I (COI) gene. This technique can be applied for use in the manipulation of embryonic parthenogenetic crayfish cells.
- Subjects
CRAYFISH; CELL culture; PROCAMBARUS; EMBRYOS; CRUSTACEAN genetics; BIOLOGICAL models
- Publication
Journal of Crustacean Biology, 2019, Vol 39, Issue 6, p758
- ISSN
0278-0372
- Publication type
Article
- DOI
10.1093/jcbiol/ruz063