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- Title
L(+) and D(-) Lactate Are Increased in Plasma and Urine Samples of Type 2 Diabetes asMeasured by a Simultaneous Quantification of L(+) and D(-) Lactate by Reversed-Phase Liquid Chromatography Tandem Mass Spectrometry.
- Authors
Scheijen, Jean L. J. M.; Hanssen, Nordin M. J.; Van de Waarenburg, Marjo P. H.; Jonkers, Daisy M. A. E.; Stehouwer, Coen D. A.; Schalkwijk, Casper G.
- Abstract
Background. Plasma and urinary levels of D-lactate have been linked to the presence of diabetes. Previously developed techniques have shown several limitations to further evaluate D-lactate as a biomarker for this condition. Methods. D- and L-lactate were quantified using ultraperformance liquid chromatography tandem mass spectrometry with labelled internal standard. Samples were derivatized with diacetyl-L-tartaric anhydride and separated on a C18-reversed phase column. D- and L-lactate were analysed in plasma and urine of controls, patients with inflammatory bowel disease (IBD), and patients with type 2 diabetes (T2DM). Results. Quantitative analysis of D- and L-lactate was achieved successfully. Calibration curves were linear (r ²0 99) over the physiological and pathophysiological ranges. Recoveries for urine and plasma were between 96% and 113%. Inter- and intra-assay variations were between 2% and 9%. The limits of detection of D-lactate and L-lactate in plasma were 0.7 μmol/L and 0.2 μmol/L, respectively. The limits of detection of D-lactate and L-lactate in urine were 8.1 nmol/mmol creatinine and 4.4 nmol/mmol creatinine, respectively. Plasma and urinary levels of D- and L-lactate were increased in patients with IBD and T2DMas compared with controls. Conclusion. The presented method proved to be suitable for the quantification of D- and L-lactate and opens the possibility to explore the use of D-lactate as a biomarker.
- Subjects
DIABETES; LACTATES; BIOMARKERS; TANDEM mass spectrometry; INFLAMMATORY bowel diseases
- Publication
Experimental Diabetes Research, 2012, p1
- ISSN
1687-5214
- Publication type
Article
- DOI
10.1155/2012/234812