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- Title
Conditional Expression of Parkinson's Disease-Related Mutant &agr;-Synuclein in the Midbrain Dopaminergic Neurons Causes Progressive Neurodegeneration and Degradation of Transcription Factor Nuclear Receptor Related 1.
- Authors
Xian Lin; Parisiadou, Loukia; Sgobio, Carmelo; Guoxiang Liu; Jia Yu; Lixin Sun; Hoon Shim; Xing-Long Gu; Jing Luo; Cai-Xia Long; Jinhui Ding; Mateo, Yolanda; Sullivan, Patricia H.; Ling-Gang Wu; Goldstein, David S.; Lovinger, David; Huaibin Cai
- Abstract
&agr;-Synuclein (&agr;-syn) plays a prominent role in the degeneration of midbrain dopaminergic (mDA) neurons in Parkinson's disease (PD). However, only a few studies on &agr;-syn have been performed in the mDA neurons in vivo, which may be attributed to a lack of &agr;-syn transgenic mice that develop PD-like severe degeneration of mDA neurons. To gain mechanistic insights into the &agr;-syn-induced mDA neurodegeneration, we generated a new line of tetracycline-regulated inducible transgenic mice that overexpressed the PD-related&agr;-syn A53T missense mutation in the mDA neurons. Here we show that the mutant mice developed profound motor disabilities and robust mDA neurodegeneration, resembling some key motor and pathological phenotypes of PD. We also systematically examined the subcellular abnormalities that appeared in the mDA neurons of mutant mice and observed a profound decrease of dopamine release, the fragmentation of Golgi apparatus, and the impairments of autophagy/lysosome degradation pathways in these neurons. To further understand the specific molecular events leading to the&agr;-syn-dependent degeneration ofmDAneurons, we found that overexpression of &agr;-syn promoted a proteasome-dependent degradation of nuclear receptor-related 1 protein (Nurr1), whereas inhibition of Nurr1 degradation ameliorated the &agr;-syn-induced loss of mDA neurons. Given that Nurr1 plays an essential role in maintaining the normal function and survival of mDA neurons, our studies suggest that the &agr;-syn-mediated suppression of Nurr1 protein expression may contribute to the preferential vulnerability of mDA neurons in the pathogenesis of PD.
- Subjects
PARKINSON'S disease; SYNUCLEINS; DOPAMINERGIC neurons; INNERVATION of the brain; NEURODEGENERATION; MISSENSE mutation; TRANSCRIPTION factors; NUCLEAR receptors (Biochemistry)
- Publication
Journal of Neuroscience, 2012, Vol 32, Issue 27, p9248
- ISSN
0270-6474
- Publication type
Article
- DOI
10.1523/JNEUROSCI.1731-12.2012