We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Improving initiation, genotype capture, and family representation in somatic embryogenesis of Pinus radiata by a combination of zygotic embryo maturity, media, and explant preparation.
- Authors
Hargreaves, Cathy L.; Reeves, Cathie B.; Find, Jens I.; Gough, Keiko; Josekutty, Puthiyaparambil; Skudder, Dolina B.; van der Maas, Susan A.; Sigley, Margaret R.; Menzies, Mike I.; Low, Charlie B.; Mullin, Tim J.
- Abstract
The principal aim of this investigation was to improve somatic embryogenesis initiation and to enhance representation of families and genotypes within those families of Pinus radiata D. Don. A total of 19 open-pollinated seed families, many with unrelated and weakly related parents, were tested. Optimum stage of cone maturity for initiation success was tested by five collections made at 1 week intervals, spanning the developmental period from pro-embryo to cotyledonary embryos. Two media were compared; embryo-development media (EDM6) and a modified Litvay medium (Glitz). Two zygotic embryo explant-preparation techniques were tested; embryos with retained megagametophytes and excised embryos. Proliferating embryogenic tissues were obtained from all four treatments (2850 explants per treatment, 570 per collection time) for the 19 families. The best initiation rates were achieved with a combination of Glitz medium with excised zygotic embryos, with 55% of explants from all collections and all families combined giving rise to proliferating embryogenic tissue. At the optimal collection time for each of the families, this treatment gave a range of 47%–97% initiation success with an average of 70% per family.
- Subjects
SEEDS; SOMATIC embryogenesis; PINUS radiata; PLACENTA; EMBRYOS; EMBRYOLOGY; PLANT embryology; PLANT tissue culture; ECOLOGICAL genetics; POLLINATION; PLANT propagation
- Publication
Canadian Journal of Forest Research, 2009, Vol 39, Issue 8, p1566
- ISSN
0045-5067
- Publication type
Article
- DOI
10.1139/X09-082