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- Title
A novel method for intratracheal injection of infectious agents into mice.
- Authors
Yong Cheng; Liepert, Lucas D.; Waller, Justin; Leevy, W. Matthew; Schorey, Jeff S.
- Abstract
Intratracheal injection is a traditional technique used in small animal studies of highly contagious airborne pathogens such as Mycobacterium tuberculosis. However, current techniques of intratracheal injection generally involve procedures that pose a risk of incident injury and infection for researchers, and may also cause collateral damage to experimental animals during the installation process. Here we describe an intratracheal injection method that was enabled by a three dimensional printing of a custom platform. This updated technique improved the overall ergonomics of intratracheal injection in mice, minimizing the risk of human injury and implementing the 3R (replacement, reduction and refinement) principle in mouse infection studies.
- Subjects
INTRATRACHEAL anesthesia; MYCOBACTERIUM tuberculosis; LABORATORY rats; MYCOBACTERIUM bovis; DIAGNOSIS; THERAPEUTICS
- Publication
Laboratory Animals, 2017, Vol 51, Issue 5, p530
- ISSN
0023-6772
- Publication type
Article
- DOI
10.1177/0023677216684422