We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Gene Expression Profiles Analysis of the Growing Rat Liver in Response to Different Zinc Status by cDNA Microarray Analysis.
- Authors
J. Y. Sun; J. F. Wang; N. T. Zi; M. Y. Jing; X. Y. Weng
- Abstract
The effects of zinc on growing rats were characterized using the dietaryzinc-deficient (ZD) and Zinc-overdose (ZO) models. Zinc deficiency had negativeeffects on the host final body weight and liver zinc content, whereas zincoverdose had positive effects. In order to identify the molecular changes inthe liver responding to dietary zinc status, cDNA microarrays were used toanalyze the expression pattern of 9753 genes in the livers of rats fed ZD andZO diet for 6 wk, compared with zinc-adequate ZA. The mRNA levels for 62genes were affected significantly by the ZD diet, whereas 66 gene transcriptionswere markedly changed in the ZO diet. Those predominant gene productsinvolved in nitrogen metabolism (glutaminase), carbohydratemetabolism (aldolase), lipid metabolism (stearoyl-CoA desaturase), growth(insulin-like growth factor-binding protein), transcription and translation(zinc-finger protein), immune (natural-killer cell), signal transduction (mitogen-activated protein kinase), and ion transportation (ATPase Naﲯ transportingpeptide) were clustered. In conclusion, a number of mammaliangenes related to zinc in the liver were identified. The characterization of thegenes and their products will allow a more comprehensive analysis of the roleof zinc in metabolism. Furthermore, the mRNA identified could be useful inestablishing the mechanisms of zinc in the pleiotropic metabolisms in vivo.
- Subjects
ZINC; GENE expression; LIVER; DNA microarrays; RATS
- Publication
Biological Trace Element Research, 2007, Vol 115, Issue 2, p169
- ISSN
0163-4984
- Publication type
Article
- DOI
10.1007/BF02686028