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- Title
Anterograde-propagation of axonal degeneration in the visual system of wlds mice characterized by diffusion tensor imaging.
- Authors
Sun, Shu‐Wei; Nishioka, Christopher; Chung, Chen‐Fang; Park, JoAnn; Liang, Hsiao‐Fang; Sun, Shu-Wei; Chung, Chen-Fang; Liang, Hsiao-Fang
- Abstract
<bold>Purpose: </bold>To evaluate the feasibility of using diffusion tensor imaging (DTI) to characterize the temporospatial profile of axonal degeneration and its relation to blood-brain barrier (BBB) permeability.<bold>Materials and Methods: </bold>Longitudinal DTI was performed in Wallerian degeneration slow (WldS) mice following retinal ischemia. In parallel, gadolinium (Gd)-enhanced T1 -weighted imaging (Gd-T1 WI) was performed to evaluate BBB permeability in white matter during axonal degeneration. To confirm the in vivo findings, immunohistochemistry using SMI-31 and myelin basic protein (MBP) was performed to examine the axons and myelin, respectively, and Evans blue was used to evaluate the permeability of the BBB.<bold>Results: </bold>Reduced axial diffusivity was found in the optic nerve (ON, -15%, P = 0.0063) 1 week and optic tact (OT, -18%, P = 0.0077) 2 weeks after retinal ischemia, which were respectively associated with an 11% (P = 0.0116) and 25% (P = 0.0001) axonal loss. Increased radial diffusivity was found 1-2 weeks after the colocated decrease of axial diffusivity (35% increase, P = 0.0388 in the ON at week 2 and an 80% increase, P = 0.0015 in the OT at week 4). No significant changes were observed using Gd-T1 WI (P = 0.13-0.75), although an approximately 1-fold increase in Evans blue staining intensity was found in the injured ON and OT starting 1 week after retinal ischemia.<bold>Conclusion: </bold>We demonstrated the utility of DTI to characterize anterograde-propagating axonal degeneration through the ON and OT following retinal damage. Evans blue staining revealed serum albumin accumulation at injured sites, although there was no BBB leakage detectable using Gd-T1 WI.<bold>Level Of Evidence: </bold>2 J. Magn. Reson. Imaging 2017;45:482-491.
- Subjects
ANIMAL experimentation; BLOOD-brain barrier; BRAIN injuries; MAGNETIC resonance imaging; MICE; NEURODEGENERATION; NEURONS; RESEARCH evaluation; RESEARCH funding; RETINAL diseases; PILOT projects; NEURAL pathways
- Publication
Journal of Magnetic Resonance Imaging, 2017, Vol 45, Issue 2, p482
- ISSN
1053-1807
- Publication type
journal article
- DOI
10.1002/jmri.25368