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- Title
Thiol- and disulfide-modified oligonucleotide monolayer structures on polycrystalline and single-crystal Au(111) surfaces.
- Authors
Hainer Wackerbarth; Rodolphe Marie; Mikala Grubb; Jingdong Zhang; AllanG. Hansen; Ib Chorkendorff; ClausB.V. Christensen; Anja Boisen; Jens Ulstrup
- Abstract
We provide a comprehensive study of single- (ss) and double-strand (ds) oligonucleotides with either 25 or 10 bases or base pairs (bp) immobilized on polycrystalline and single-crystal Au(111) surfaces. The study is based on X-ray photoelectron spectroscopy, cyclic and differential pulse voltammetry, interfacial capacitance data, and electrochemical scanning tunnelling microscopy (in situ STM). The sequences used were the 25-bp sequence from the BRCA1 gene (25-mer), while the 10-bp oligonucleotides contained solely linear adenine and thymine sequences. The oligonucleotides were modified by the dimethoxytrityl group (DMT) via a disulfide group [DMT-S-S-ss25-mer and DMT-S-S-ds(AT) 10], a pure disulfide group (A 10-S-S-T 10), or a thiol group [HS-ss25-mer and HS-ds-(AT) 10], all via a hexamethylene linker. The overall pattern suggests strategies for controlled adsorption of DNA-based molecules and recognition of complementary strands or other molecules.
- Subjects
OLIGONUCLEOTIDES; SPECTRUM analysis; MOLECULES; ELECTRON spectroscopy
- Publication
Journal of Solid State Electrochemistry, 2004, Vol 8, Issue 7, p474
- ISSN
1432-8488
- Publication type
Article
- DOI
10.1007/s10008-003-0466-9