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- Title
Distribution and role of tenascin-C in human osteoarthritic cartilage.
- Authors
Nakoshi, Yutaka; Hasegawa, Masahiro; Akeda, Koji; Iino, Takahiro; Sudo, Akihiro; Yoshida, Toshimichi; Uchida, Atsumasa
- Abstract
Background: Tenascin-C (TN-C) is expressed in the cartilage of osteoarthritis (OA). We examined whether TN-C was involved in cartilage repair of the diseased joints. Human articular cartilage samples were obtained from patients with OA and those with normal joints. Methods: Immunohistochemistry testing of TN-C, chondroitin sulfate (CS), and proliferating cell nuclear antigen (PCNA) was performed. Chondrocytes were isolated from human cartilage and cultured. After treatment with TN-C, chondrocyte proliferation s was analyzed by bromodeoxyuridine (BrdU) incorporation assay using an enzyme-linked immunosorbent assay kit. Glycosaminoglycan content was determined by dimethylmethylene blue (DMMB) assay. The mRNA expression of aggrecan was also analyzed, by quantitative real-time polymerase chain reaction (PCR). Results: In osteoarthritic cartilage, increased TN-C staining was observed with the degeneration of articular cartilage in comparison with normal cartilage. TN-C staining was shown in the cartilage surface overlying CS-positive areas. In addition, the expression of PCNA in the positive areas for TN-C was significantly higher than that in the negative areas. Treatment of human articular chondrocytes with 10 μg/ml TN-C accelerated chondrocyte proliferation, increased the proteoglycan amount in culture, and increased the expression of aggrecan mRNA. Conclusions: Our findings indicate that the distribution of TN-C is related to CS production and chondrocyte proliferation in osteoarthritic cartilage and that TN-C has effects on DNA synthesis, proteoglycan content, and aggrecan mRNA expression in vitro. TN-C may be responsible for repair in human osteoarthritic cartilage.
- Subjects
OSTEOARTHRITIS; CARTILAGE cells; TENASCIN; IMMUNOHISTOCHEMISTRY; CHONDROITIN sulfates; CELL proliferation; ENZYME-linked immunosorbent assay; DNA synthesis; GENE expression
- Publication
Journal of Orthopaedic Science, 2010, Vol 15, Issue 5, p666
- ISSN
0949-2658
- Publication type
Article
- DOI
10.1007/s00776-010-1513-x