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- Title
Effects of High Intensity Ultrasound on Physiochemical and Structural Properties of Goat Milk β-Lactoglobulin.
- Authors
Zhou, Xinhui; Wang, Cuina; Sun, Xiaomeng; Zhao, Zixuan; Guo, Mingruo
- Abstract
This study aimed to compare the effects of high intensity ultrasound (HIU) applied at various amplitudes (20~40%) and for different durations (1~10 min) on the physiochemical and structural properties of goat milk β-lactoglobulin. No significant change was observed in the protein electrophoretic patterns by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Deconvolution and second derivative of the Fourier transform infrared spectra (FTIR) showed that the percentage of β-sheet of goat milk β-lactoglobulin was significantly decreased while those of α-helix and random coils increased after HIU treatment The surface hydrophobicity index and intrinsic fluorescence intensity of samples was enhanced and increased with increasing HIU amplitude or time. Differential scanning calorimetry (DSC) results exhibited that HIU treatments improved the thermal stability of goat milk β-lactoglobulin. Transmission electron microscopy (TEM) of samples showed that the goat milk β-lactoglobulin microstructure had changed and it contained larger aggregates when compared with the untreated goat milk β-lactoglobulin sample. Data suggested that HIU treatments resulted in secondary and tertiary structural changes of goat milk β-lactoglobulin and improved its thermal stability.
- Subjects
GOAT milk; GOATS; SODIUM dodecyl sulfate; POLYACRYLAMIDE gel electrophoresis; DIFFERENTIAL scanning calorimetry; TRANSMISSION electron microscopy
- Publication
Molecules, 2020, Vol 25, Issue 16, p3637
- ISSN
1420-3049
- Publication type
Article
- DOI
10.3390/molecules25163637