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- Title
Ectopic Overexpression of a Novel R2R3-MYB, NtMYB2 from Chinese Narcissus Represses Anthocyanin Biosynthesis in Tobacco.
- Authors
Anwar, Muhammad; Wang, Guiqing; Wu, Jiacheng; Waheed, Saquib; Allan, Andrew C.; Zeng, Lihui
- Abstract
R2R3 MYB transcription factors play key functions in the regulation of secondary metabolites. In the present study, a R2R3 MYB transcriptional factor <italic>NtMYB2</italic> was identified from Chinese narcissus (<italic>Narcissus tazetta</italic> L. var. <italic>Chinensis</italic> Roem) and functionally characterized. <italic>NtMYB2</italic> belongs to subgroup 4 of the R2R3 MYB transcription factor family that are related to repressor MYBs involved in the regulation of anthocyanin and flavonoids. Transient expression confirmed that <italic>NtMYB2</italic> strongly reduced the red pigmentation induced by MYB- anthocyanin activators in agro-infiltrated tobacco leaves. Ectopic expression of <italic>NtMYB2</italic> in tobacco significantly reduced the pigmentation and altered the floral phenotypes in transgenic tobacco flowers. Gene expression analysis suggested that <italic>NtMYB2</italic> repressed the transcript levels of structural genes involved in anthocyanin biosynthesis pathway, especially the <italic>UFGT</italic> gene. <italic>NtMYB2</italic> gene is expressed in all examined narcissus tissues; the levels of transcription in petals and corona is higher than other tissues and the transcription level at the bud stage was highest. These results show that <italic>NtMYB2</italic> is involved in the regulation of anthocyanin biosynthesis pathway and may act as a repressor by down regulating the transcripts of key enzyme genes in Chinese narcissus.
- Subjects
TRANSCRIPTION factors; METABOLITES; ANTHOCYANINS; FLAVONOIDS; GENE expression; BIOSYNTHESIS
- Publication
Molecules, 2018, Vol 23, Issue 4, p781
- ISSN
1420-3049
- Publication type
Article
- DOI
10.3390/molecules23040781