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- Title
First detection of wheat streak mosaic virus in Germany: molecular and biological characteristics.
- Authors
Schubert, Jörg; Ziegler, Angelika; Rabenstein, Frank
- Abstract
Wheat streak mosaic virus is a serious threat in wheat-producing countries. In Germany, the virus was first recorded in 2013 near Hoym. The complete sequence of isolate Hoym was obtained and compared to all other known complete WSMV sequences, including newly collected and sequenced isolates from France and Austria. Phylogenetic analysis revealed that the European isolates group together with those from the Middle East to form a separate cluster characterized by a distinct putative P1 protease cleavage site. By means of quantitative reverse transcription polymerase chain reaction, it was shown that RNA of the USA type strain PV57 accumulated to higher levels in infected wheat cv. Alcedo than did RNA of isolate Hoym.
- Subjects
GERMANY; WHEAT streak mosaic virus; CLADISTIC analysis; PROTEOLYTIC enzymes; REVERSE transcriptase polymerase chain reaction; SEQUENCE analysis; VIRAL genomes; RNA sequencing
- Publication
Archives of Virology, 2015, Vol 160, Issue 7, p1761
- ISSN
0304-8608
- Publication type
Report
- DOI
10.1007/s00705-015-2422-2