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- Title
Genome-wide identification and analysis of the U-box family of E3 ligases in grapevine.
- Authors
Yu, Y.; Li, X.; Guo, D.; Zhang, H.; Li, G.; Zhang, G.
- Abstract
E3 ubiquitin ligases play essential roles in determining the specificity of ubiquitination and subsequent protein degradation. The plant U-box ( PUB) family of E3 ligases has been implicated in biotic and abiotic stress signaling and developmental events in various species. A comprehensive bioinformatics analysis identified 56 PUB genes in the grapevine ( Vitis vinifera L.) genome. Based on conserved motifs, the VvPUB family was classified into seven subclasses. Expansion of this family was driven by chromosomal, segmental, and tandem duplications. Microarray expression profiling revealed that three PUB genes were preferentially expressed in pollen, four in leaf, and five in root. Moreover, a large number of PUB genes were differentially expressed under abiotic stresses and eight PUB genes likely participated in defense against powdery mildew. The microarray expression data were verified by RT-qPCR. Genome-wide identification of VvPUB genes and examination of their expression will give insights into the functions of U-box genes in grapevine.
- Subjects
UBIQUITIN ligases; GRAPES; PROTEOLYSIS; UBIQUITINATION; ABIOTIC stress; PLANT development
- Publication
Russian Journal of Plant Physiology, 2016, Vol 63, Issue 6, p835
- ISSN
1021-4437
- Publication type
Article
- DOI
10.1134/S1021443716050186