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- Title
Enzymatic synthesis of l-norephedrine by coupling recombinant pyruvate decarboxylase and ω-transaminase.
- Authors
Wu, Xuri; Fei, Mengdan; Chen, Yong; Wang, Zongqiang; Chen, Yijun
- Abstract
l-Norephedrine, a natural plant alkaloid, possesses similar activity as ephedrine and can be used as a vicinal amino alcohol for the asymmetric synthesis of a variety of optically pure compounds, including pharmaceuticals, fine chemicals, and agrochemicals. Because of the existence of two asymmetric centers, efficient synthesis of l-norephedrine has been challenging. In the present study, an R-selective pyruvate decarboxylase from Saccharomyces cerevisiae and an S-selective ω-transaminase from Vibrio fluvialis JS17 were coupled to develop a sequential process for the stereoselective biosynthesis of l-norephedrine. After systematic optimization of the reaction conditions, a green, economic, and practical biocatalytic method to prepare l-norephedrine was established to achieve de and ee values of greater than 99.5 % and a molar yield over 60 %. The present coupling approach can facilitate the development of sequential reactions by various biocatalysts.
- Subjects
PYRUVATE decarboxylase; PHENYLPROPANOLAMINE; BIOSYNTHESIS; ASYMMETRIC synthesis; SACCHAROMYCES cerevisiae; AMINOTRANSFERASES
- Publication
Applied Microbiology & Biotechnology, 2014, Vol 98, Issue 17, p7399
- ISSN
0175-7598
- Publication type
Article
- DOI
10.1007/s00253-014-5797-1