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- Title
300 Impact of Fiber-Degrading Enzymes on Microbial Composition and NSP Metabolites in Nursery Pigs Fed a Higher Fiber Diet.
- Authors
Li, Q Y; Schmitz-Esser, S; Patience, J F
- Abstract
The addition of fiber-degrading enzymes to high fiber diets may modulate intestinal microbial populations and non-starch polysaccharide (NSP) metabolites due to fiber degradation. This experiment was conducted to evaluate the effects of dietary xylanase (X; Huvepharma Inc.) and an enzyme blend (EB: cellulase, ß-glucanase, and xylanase; Huvepharma Inc.) on intestinal microbial populations and metabolites of weaned piglets fed a higher fiber diet. A total of 460 pigs (6.43 ± 0.06 kg BW; F25 Genetiporc×6.0 Genetiporc) were blocked by weight and randomly assigned to 4 treatments, in a 2 × 2 factorial arrangement: X (0 vs 0.01%) and EB (0 vs 0.01%). There were 12 blocks and 48 pens. Diets were based on corn, soybean meal plus corn DDGS and wheat middlings at 5 or 10% each for wk 1-2 or 3-4, respectively. Digesta was collected on d 28 to determine microbial populations and fermentation products. Total bacteria 16S rRNA gene copy numbers were quantified using qPCR. Specific bacterial relative abundance was expressed as a proportion of total bacteria. Data were analyzed using PROC MIXED (SAS, 9.4) with pen as the experimental unit. Xylanase, EB and X*EB interaction were considered fixed effects. No interactions between X and EB on cecal VFA concentration were observed. The EB decreased (<italic>P</italic><0.05) cecal propionate, butyrate, valerate, and total VFA (129.52 vs. 142.94 μM/g) compared with no EB. Xylanase had no effect on any VFA in the cecum. In the colon, X increased isobutyrate and isovalerate concentrations (<italic>P</italic><0.05) compared to diets without X. There was an X*EB interaction for colonic propionate concentration; in the absence of xylanase, EB decreased propionate compared to control (37.03 vs. 44.85 μM/g; <italic>P</italic><0.05), but in the presence of xylanase, EB did not affect colonic propionate (X vs. X+EB; P>0.10). An X*EB interaction trend was also detected for colonic butyrate and total VFA; when xylanse was not present, EB tended to reduce their concentrations compared to control (<italic>P</italic><0.10), whereas, when xylanase was present, EB had no impact (X vs. X+EB; P>0.10). The EB tended to reduce the relative abundance of <italic>Enterobacteriaceae</italic> family (<italic>P</italic><0.10). Unexpectedly, EB reduced the relative abundance of <italic>Lactobacillus spp.</italic> (19.17 vs.27.12%; P<0.05) in the colon. In summary, EB decreased total VFA concentration in the cecum and relative abundance of <italic>Lactobacillus spp.</italic> in the colon. This might indicate that pigs fed EB diets had less substrate for microbes in the large intestine compared to those fed diets without EB.
- Subjects
SWINE nutrition; FIBER in animal nutrition; MICROBIAL metabolites; XYLANASES; POLYSACCHARIDES; GUT microbiome
- Publication
Journal of Animal Science, 2018, Vol 96, p161
- ISSN
0021-8812
- Publication type
Article
- DOI
10.1093/jas/sky073.297