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- Title
Expression of a-tocopherol-associated genes and a-tocopherol accumulation in Japanese Black (Wagyu) calves with and without a-tocopherol supplementation.
- Authors
Haga, S.; Nakano, M.; Ishizaki, H.; Roh, S. G.; Katoh, K.
- Abstract
The aim of the study was to clarify 1) the distribution of 6 a-tocopherol (a-Toc)-associated gene expressions in 20 major tissues, including metabolic, reproductive, endocrine, immune, and digestive and absorptive tissues, in relation to a-Toc status and 2) the change in expression patterns of the genes induced when a-Toc was orally administered to Japanese Black (JB) calves. This study examined weaned male JB calves (n = 10), of which 5 calves were orally administered a-Toc for 2 wk (30 IU·kg-1 BW·d-1; TOC group). The others did not receive the a-Toc supplement and were the control (CONT) group. The 20 tissues and venous blood (serum) were sampled on the final day. In both groups, the mean mRNA expression levels for a-Toc transfer protein, afamin (AFM), ATP-binding cassette transporter A1, and tocopherol-associated protein were greatest in the liver (P < 0.05), whereas scavenger receptor class B, Type I (SR-BI) mRNA was greatest in the adrenal gland (P < 0.05). The gene for cytochrome P450 family 4, subfamily F, polypeptide 2 was most highly expressed in the liver, testes, and adrenal gland. The a-Toc content was greatest (P < 0.05) in the testes of the 20 sampled tissues in the CONT group. However, the levels in the testes and jejunum were similar and greater (P < 0.05) than the levels in the other 18 tissues in the TOC group. The mean increase in a-Toc levels after oral a-Toc administration (mean a-Toc content for the TOC group divided by the CONT group content) were greater (P < 0.05) in the jejunum (40.7- fold) and duodenum and liver (26.3- and 23.1-fold) than in the serum (7.8-fold). In the liver, a-Toc administration significantly increased (P < 0.05) the AFM and SR-BI mRNA expression levels. The results show that the liver may play an important role in the regulation of a-Toc disposition, but other peripheral tissues that accumulate large amounts of a-Toc could moderate the local a-Toc status and functions, as inferred from the high expressions of the a-Toc-associated genes in JB calves.
- Subjects
GENE expression; VITAMIN E in animal nutrition; CALVES; CATTLE nutrition; DIETARY supplements; CARRIER proteins; MESSENGER RNA
- Publication
Journal of Animal Science, 2015, Vol 93, Issue 8, p4048
- ISSN
0021-8812
- Publication type
Article
- DOI
10.2527/jas.2015-9106