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- Title
薏苡黑粉菌基因组测序揭示其物种分类及适应性 进化.
- Authors
李祥栋; 郑传奇; 陆秀娟; 潘虹; 魏心元; 石明; 付瑜华; 陆平; 高爱农; 周美亮
- Abstract
Affected tissues of adlay (Coix L.) were selected as research materials and the adlay smut Ustilago coicis were separated, purified and observed in solid and liquid nutrient medium. The smut fungus genome were sequenced, assembled and annotated by Illumina, Pacbio and Hi-C technologies. The homology gene family evolution and phylogeny were studied by comparative genetics among adlay smut fungus and the other six smut fungus species. Ustilago coicis was successfully cultured and purified in solid PDA and liquid PDB media at pH 7. The colony on PDA is pulvinate and wrinkly, with white color, moist and opaque. Illumina, Pacbio and Hi-C sequencing and assembly showed that the whole genome of adlay smut fungus has 20 chromosome pairs (2n=40), with a size of 20.093 417 Mb and 53.81% GC content. There were a prediction and annotation of 7 476 protein-coding and 164 non-coding genes with a length ratio of 61.02% and 0.285 3% respectively. A sum of 3 674 dispersed duplications and 8 139 tandem duplications was hunted, occupying 2.754 4% and 1.605 4% of the whole genome length. Homology gene clustering exhibited that protein-coding genes of U. coicis, U. bromivora, U. maydis, U. hordei, Sporisorium reilianum, S. scitamineum, and S. graminicola were clustered into 6 999 gene families in which shared gene families totaled 5 379. There were 5 339 single-copy orthologs, 105 multiple-copy orthologs, 89 unique paralogs, 752 other othologs and 188 unclustered genes in U. coicis. The gene family evolution and phylogeny clustering also revealed that U. coicis was split in ~60.9 MYA, and clustered with U. bromivova and U. hordei, hinting their closer relatives; however, U. maydis, a species parasitized corn, was split in ~66.6 MYA, and clustered with S. scitamineum, S. graminicola and S. reilianum.
- Subjects
COMPARATIVE genetics; GENE families; GENE clusters; PHYLOGENY; CHROMOSOMES
- Publication
Mycosystema, 2022, Vol 41, Issue 12, p1960
- ISSN
1672-6472
- Publication type
Article
- DOI
10.13346/j.mycosystema.220079