We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
SUMOylation of nuclear actin.
- Authors
Hofmann, Wilma A.; Arduini, Alessandro; Nicol, Samantha M.; Camacho, Carlos J.; Lessard, James L.; Fuller-Pace, Frances V.; de Lanerolle, Primal
- Abstract
Actin, a major component of the cytoplasm, is also abundant in the nucleus. Nuclear actin is involved in a variety of nuclear processes including transcription, chromatin remodeling, and intranuclear transport. Nevertheless, the regulation of nuclear actin by posttranslational modifications has not been investigated. We now show that nuclear actin is modified by SUMO2 and SUMO3 and that computational modeling and site-directed mutagenesis identified K68 and K284 as critical sites for SUMOylating actin. We also present a model for the actin-SUMO complex and show that SUMOylation is required for the nuclear localization of actin.
- Subjects
ACTIN; GENETIC regulation; UBIQUITIN; PROTEINS; GENETIC translation; MUTAGENESIS
- Publication
Journal of Cell Biology, 2009, Vol 186, Issue 2, p193
- ISSN
0021-9525
- Publication type
Article
- DOI
10.1083/jcb.200905016