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- Title
Regulation of a formin complex by the microtubule plus end protein tea1p.
- Authors
Feierbach, Becket; Fulvia Verde; Fred Chang
- Abstract
The plus ends of microtubules have been speculated to regulate the actin cytoskeleton for the proper positioning of sites of cell polarization and cytokinesis. In the fission yeast Schizosaccharomyces pombe, interphase microtubuies and the kelch repeat protein tea1p regulate polarized cell growth. Here, we show that tea1p is directly deposited at cell tips by microtubule plus ends. Tea1p associates in large "polarisome" complexes with bud6p and for3p, a formin that assembles actin cables. Tea1 p also interacts in a separate complex with the CLIP-170 protein tip1p, a microtubule plus end-binding protein that anchors teal p to the microtubule plus end. Localization experiments suggest that tea1 p and bud6p regulate formin distribution and actin cable assembly. Although single mutants still polarize, for 3Δbud6Δtea 1Δ triple-mutant cells lack polarity, indicating that these proteins contribute overlapping functions in cell polarization. Thus, these experiments begin to elucidate how microtubules contribute to the proper spatial regulation of actin assembly and polarized cell growth.
- Subjects
MICROTUBULES; ORGANELLES; ACTIN; ACTOMYOSIN; CYTOKINESIS; CELL division; CYTOPLASM
- Publication
Journal of Cell Biology, 2004, Vol 165, Issue 5, p697
- ISSN
0021-9525
- Publication type
Article
- DOI
10.1083/jcb.200403090