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- Title
Comparison of Four SARS-CoV-2 Neutralization Assays.
- Authors
Riepler, Lydia; Rössler, Annika; Falch, Albert; Volland, André; Borena, Wegene; von Laer, Dorothee; Kimpel, Janine
- Abstract
Neutralizing antibodies are a major correlate of protection for many viruses including the novel coronavirus SARS-CoV-2. Thus, vaccine candidates should potently induce neutralizing antibodies to render effective protection from infection. A variety of in vitro assays for the detection of SARS-CoV-2 neutralizing antibodies has been described. However, validation of the different assays against each other is important to allow comparison of different studies. Here, we compared four different SARS-CoV-2 neutralization assays using the same set of patient samples. Two assays used replication competent SARS-CoV-2, a focus forming assay and a TCID50-based assay, while the other two assays used replication defective lentiviral or vesicular stomatitis virus (VSV)-based particles pseudotyped with SARS-CoV-2 spike. All assays were robust and produced highly reproducible neutralization titers. Titers of neutralizing antibodies correlated well between the different assays and with the titers of SARS-CoV-2 S-protein binding antibodies detected in an ELISA. Our study showed that commonly used SARS-CoV-2 neutralization assays are robust and that results obtained with different assays are comparable.
- Subjects
SARS-CoV-2; VESICULAR stomatitis; VITRONECTIN; ANTIBODY titer
- Publication
Vaccines, 2021, Vol 9, Issue 1, p13
- ISSN
2076-393X
- Publication type
Article
- DOI
10.3390/vaccines9010013