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- Title
Analysis of lncRNA expression and regulation network in postmenopausal osteoporosis patients with Kidney Yin deficiency.
- Authors
XU Huijuan; CHEN Juan; LI Shengqiang; HU Zhixu; GE Jirong; XIE Lihua
- Abstract
Objective To investigate the lncRNA expression and regulation network in postmenopausal osteoporosis with kidney Yin deficiency. Methods The patients with postmenopausal osteoporosis were selected and randomly divided into: kidney Yin deficiency group (n = 3) and kidney Yang deficiency group (n = 3). Three healthy postmenopausal women were selected as control group. lncRNA gene expression in single nuclear cells in peripheral blood was examined using microarray method. The genes in kidney Yin deficiency group were compared to those in other 2 groups respectively. Common differential expressed genes were studied and the regulation network was established. Real-time RT-PCR was used to examine the expression of LINC00334, LINC00189, and LOC101929378 and the results of microarray were confirmed. Results Eight differential expressed lncRNAs, LINC00334 LINC00189, LOC101929378, XLOC _ 013921, ENSG00000237489. 2, ENSG00000225278. 2, AK125437, and RNA95672 were screened out from the comparison among the 3 groups. Pathway analysis indicated that 12 potential pathways corresponded to these aberrant lincRNAs. Real-time PCR confirmed that LINC00189 was down regulated in the kidney Yin deficiency group (FC = 4. 71, P = 0. 007). LINC00334 (FC = 6. 83, P = 0. 005) and LOC101929378 (FC = 4. 51, P = 0. 035 ) were up-regulated in the kidney Yin deficiency group. The change trend was coincident with the result of CHIP. Conclusion Eight lncRNAs, LINC00334, LINC00189, LOC101929378, XLOC_013921, ENSG00000237489. 2, ENSG00000225278. 2, AK125437, and RNA95672, are involved in osteoporotic kidney Yin deficiency by regulation of Jak/Stat, MAPK, chemokine signaling pathway, and calcium signaling pathway in postmenopausal osteoporosis.
- Publication
Chinese Journal of Osteoporosis, 2015, Vol 21, Issue 5, p553
- ISSN
1006-7108
- Publication type
Article
- DOI
10.3969/j.issn.1006-7108.2015.05.008