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- Title
雷公藤甲素诱导肝细胞选择性自噬的形态学观察.
- Authors
蒋杨雨; 江始源; 曹 丹; 王海燕; 邹昊靖; 李 健
- Abstract
Objective: To observe the level of hepatocyte selective autophagy induced by triptolide. Methods: An autophagy tracing model in hepatocytes was established by rapid tail vein injection of GFP-LC3 plasmids into mice treated with triptolide to observe the hepatocyte autophagy level induced by triptolide in vivo. GFP-LC3 stable expressed L02 cells were transfected with RFP-P62 plasmid, and the selective autophagy profile of L02 and LC3-P62 complex induced by triptolide were dynamically observed by live cell imaging system and fluorescence microscopy. Results: The results of in vivo experiments showed that triptolide significantly induced autophagy in hepatocytes. And a similar trend on the protein expression of LC3II and P62 was demonstrated by Western blot. The results of live cell imaging system and fluorescence microscopy showed the co-localization of LC3 and P62 suggesting that the triptolide could induce autophagy flux. Conclusion: Triptolide induced selective autophagy in hepatocytes and its biological function might be associated with hepatocyte damage repairing after injury.
- Subjects
CELL imaging; IMAGING systems; FLUORESCENCE microscopy; TRIPTOLIDE; PROTEIN expression
- Publication
Progress in Modern Biomedicine, 2023, Vol 23, Issue 2, p206
- ISSN
1673-6273
- Publication type
Article
- DOI
10.13241/j.cnki.pmb.2023.02.002