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- Title
Optimisation of High-Performance Thin-Layer Chromatography-Based Antioxidant Profiling of Caulerpa lentillifera Extracts.
- Authors
Hamli, Aiman; Ramasamy, Kalavathy; Agatonovic-Kustrin, Snezana; Shah, Syed Adnan Ali; Rahim, Nur Syafiqah; Siong Meng Lim
- Abstract
This study optimised a high-performance thin-layer chromatography (HPTLC) method that could facilitate the visualisation and quantification of antioxidative phytochemical compounds in Caulerpa lentillifera. This method involved the use of 2,2-diphenyl-1-picrylhydrazyl (DPPH•) and ferric chloride (FeCl3) as derivatising agents. It was found that the incubation period for DPPH• was consistent with 60 minutes in the dark, while the plate that was derivatised with FeCl3 needed to be neutralised using NH4OH and then dried at 100°C for 5 minutes. The antioxidant activity of the seaweed extracts can now be analysed using Videoscan and quantified as gallic acid equivalents (GAE).
- Subjects
CAULERPA; FERRIC chloride; GALLIC acid; IRON chlorides; THIN layer chromatography
- Publication
Malaysian Journal of Medicine & Health Sciences, 2023, Vol 19, p61
- ISSN
1675-8544
- Publication type
Article