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- Title
Effects of α-ketoglutarate on neutrophil intracellular amino and α-keto acid profiles and ROS production.
- Authors
Mühling, Jörg; Tussing, F.; Nickolaus, K. A.; Matejec, R.; Henrich, M.; Harbach, H.; Wolff, M.; Weismüller, K.; Engel, J.; Welters, I. D.; Langefeld, T. W.; Fuchs, M.; Weigand, M. A.; Heidt, M. C.
- Abstract
The aim of this study was to determine the effects of α-ketoglutarate on neutrophil (PMN), free α-keto and amino-acid profiles as well as important reactive oxygen species (ROS) produced [superoxide anion (O2−), hydrogen peroxide (H2O2)] and released myeloperoxidase (MPO) acitivity. Exogenous α-ketoglutarate significantly increased PMN α-ketoglutarate, pyruvate, asparagine, glutamine, asparatate, glutamate, arginine, citrulline, alanine, glycine and serine in a dose as well as duration of exposure dependent manner. Additionally, in parallel with intracellular α-ketoglutarate changes, increases in O2– formation, H2O2-generation and MPO acitivity have also been observed. We therefore believe that α-ketoglutarate is important for affecting PMN “susceptible free amino- and α-keto acid pools” although important mechanisms and backgrounds are not yet completely explored. Moreover, our results also show very clearly that changes in intragranulocytic α-ketoglutarate levels are relevant metabolic determinants in PMN nutrition considerably influencing and modulating the magnitude and quality of the granulocytic host defense capability as well as production of ROS.
- Subjects
NEUTROPHILS; AMINO acids; KETONIC acids; IMMUNE response; GRANULOCYTES; REACTIVE oxygen species; SUPEROXIDES
- Publication
Amino Acids, 2010, Vol 38, Issue 1, p167
- ISSN
0939-4451
- Publication type
Article
- DOI
10.1007/s00726-008-0224-5