We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Root exudates stimulate the uptake and metabolism of organic carbon in germinating spores of Glomus intraradices.
- Authors
Bücking, Heike; Abubaker, Jehad; Govindarajulu, Manjula; Tala, Marie; Pfeffer, Philip E.; Nagahashi, Gerald; Lammers, Peter; Shachar-Hill, Yair
- Abstract
• Root exudates play a key role during the presymbiotic growth phase and have been shown to stimulate hyphal branching and the catabolic metabolism of arbuscular mycorrhizal (AM) fungal spores. • Here, the effect of root exudates on presymbiotic growth, uptake of exogenous carbon and transcript levels for genes putatively involved in the carbon metabolism of germinating spores were determined. • Crude root exudates led to a slight acceleration of spore germination, increased germ tube branching and stimulated uptake and catabolic metabolism of acetate, and to a greater extent of glucose, but had no effect on gene expression. By contrast, partially purified root exudates increased the transcript levels of acyl-CoA dehydrogenase (ß-oxidation of fatty acids to acetyl-CoA), malate synthase (glyoxylate cycle) and glutamine-fructose-6-phosphate aminotransferase (chitin biosynthesis), but did not differ from crude root exudates in their effect on substrate uptake and respiration. The expression of glycogen synthase (glycogen biosynthesis), glucose-6-phosphate dehydrogenase (pentose phosphate pathway) and neutral trehalase (hydrolysis of trehalose) were only marginally or not affected by root exudates. • Root exudates have an effect on both membrane activity and gene expression and the results are discussed in relation to the catabolic and anabolic metabolism of spores during presymbiotic growth.
- Subjects
DEHYDROGENASES; VESICULAR-arbuscular mycorrhizas; CARBON; GLUTAMINE; RESPIRATION in plants; PLANT roots; PLANT exudates
- Publication
New Phytologist, 2008, Vol 180, Issue 3, p684
- ISSN
0028-646X
- Publication type
Article
- DOI
10.1111/j.1469-8137.2008.02590.x