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- Title
Candidate Cell Substrates, Vaccine Production, and Transmissible Spongiform Encephalopathies.
- Authors
Piccardo, Pedro; Cervenakova, Larisa; Vasilyeva, Irina; Yakovleva, Oksana; Bacik, Igor; Cervenak, Juraj; McKenzie, Carroll; Kurillova, Lubica; Gregori, Luisa; Pomeroy, Kitty; Asher, David M.
- Abstract
Transmissible spongiform encephalopathy (TSE) agents have contaminated human tissue-derived medical products, human blood components, and animal vaccines. The objective of this study was to determine the potential susceptibility to infection of 5 cell lines used or proposed for manufacture of biological products, as well as other lines. Cell lines were exposed to the infectious agents of sporadic and variant Creutzfeldt-Jakob disease and bovine spongiform encephalopathy (BSE). Exposed cultures were tested for TSE-associated prion protein (PrP[sup TSE]) and TSE infectivity by assay in rodents and nonhuman primates. No PrPTSE or infectivity has been detected in any exposed cell line under study so far. Animals inoculated with BSE brain homogenate developed typical spongiform encephalopathy. In contrast, animals inoculated with cells exposed to the BSE agent remained asymptomatic. All cell lines we studied resisted infection with 3 TSE agents, including the BSE agent.
- Subjects
CHRONIC wasting disease; CELLS; CELL lines; BOVINE spongiform encephalopathy; PHEOCHROMOCYTOMA; COMMUNICABLE diseases; INFECTIOUS disease transmission
- Publication
Emerging Infectious Diseases, 2011, Vol 17, Issue 12, p2262
- ISSN
1080-6040
- Publication type
Article
- DOI
10.3201/eid1712.110607