Background: Nasopharyngeal carcinoma (NPC) is a unique tumor due to its etiology and endemic distribution. Ethnic and regional factors are found to strongly influence the risk of disease; however, there have been no well-conducted studies on Indian patients. The present study assesses the relationship between Epstein–Barr Virus (EBV) and sporadic Indian NPC and the role of serum EBV DNA in NPC detection.