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- Title
The Na<sup>+</sup>/Ca<sup>2+</sup> antiporter slr0681 affects carotenoid production in Synechocystis sp. PCC 6803 under high-light stress.
- Authors
Jiaye Zhang; Huairong Zhong; Ning Xuan; Mushtaq, Rubina; Yahui Shao; Xue Cao; Pengchong Wang; Gao Chen
- Abstract
BACKGROUND: Carotenoids play key roles in photosynthesis and are widely used in foods as natural pigments, antioxidants, and health-promoting compounds. Enhancing carotenoid production in microalgae via biotechnology has become an important area of research. RESULTS: We knocked out the Na+/Ca2+ antiporter gene slr0681 in Synechocystis sp. PCC 6803 via homologous recombination and evaluated the effects on carotenoid production under normal (NL) and high-light (HL) conditions. On day 7 of NL treatment in calcium ion (Ca2+)-free medium, the cell density of Δslr0681 decreased by 29% compared to the wild type (WT). After 8 days of HL treatment, the total carotenoid contents decreased by 35% in Δslr0681, and the contents of individual carotenoids were altered: myxoxanthophyll, echinenone, and β-carotene contents increased by 10%, 50%, and 40%, respectively, while zeaxanthin contents decreased by ~40% in Δslr0681 versus the WT. The expression patterns of carotenoid metabolic pathway genes also differed: ipi expression increased by 1.2- to 8.5-fold, whereas crtO and crtR expression decreased by ~90% and 60%, respectively, in Δslr0681 versus the WT. In addition, in Δslr0681, the expression level of psaB (encoding a photosystem I structural protein) doubled, whereas the expression levels of the photosystem II genes psbA2 and psbD decreased by ~53% and 84%, respectively, compared to the WT. CONCLUSION: These findings suggest that slr0681 plays important roles in regulating carotenoid biosynthesis and structuring of the photosystems in Synechocystis sp. This study provides a theoretical basis for the genetic engineering of microalgae photosystems to increase their economic benefits and lays the foundation for developing microalgae germplasm resources with high carotenoid contents.
- Subjects
SYNECHOCYSTIS; CAROTENOIDS; ZEAXANTHIN; HOMOLOGOUS recombination; CALCIUM ions; XANTHOPHYLLS; PHOTOSYSTEMS; GERMPLASM
- Publication
Journal of the Science of Food & Agriculture, 2024, Vol 104, Issue 5, p3147
- ISSN
0022-5142
- Publication type
Article
- DOI
10.1002/jsfa.13205