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- Title
Cell block: A harvesting delight with histological power.
- Authors
Satyakiran, G. V. V.; Soumya M.; Reshma V., Sudhakara M.; Paremala K.; Radhika M. B.
- Abstract
Background: The cell block technique refers to paraffin embedding of cellular sediments. A spectrum of different cell block techniques can be used for different samples. Examining smears from fine needle aspiration (FNA) obtained from swellings of head and neck (H&N) region and small tissue fragments (STF) less than 5 mm are usually not used for histopathological examination, but have the capacity to provide adjuvant diagnostic information. But by using cell block technique with the same samples, definitive diagnosis and tumor categorization can be done. Aim: To evaluate the viability of cell block as a complementary method for the diagnosis of swellings and pathologies of head and neck region. Materials and Methods: Ten samples of FNA and 5 STF from different pathologies of H&N were collected, centrifuged at 2000 rpm for 20 min, sediment obtained was fixed in 10% formalin for 24 hours, cell block was prepared using bacterial agar method and 4 µm sections were stained and analyzed by light microscopy. Results: The different cases had distinct morphological features on cell block and they were identical to those seen in histopathological sections. Conclusion: Cell blocks should be considered in all FNA as an alternative for smear. Representative STF can also be utilized to the fullest using cell block technique. The sections obtained from cell block are comparable to histological section and can be preserved for a long duration and therefore studies using special stains, immunocytochemical and molecular biomarkers have infinite possibilities. Cell blocks also aid in the therapeutic planning of the pathology.
- Subjects
NECK tumors; HEAD tumors; CANCER cells
- Publication
Journal of Oral & Maxillofacial Pathology (0973029X), 2014, Vol 18, pS43
- ISSN
0973-029X
- Publication type
Abstract