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- Title
Outer membrane protein A of Escherichia coli forms temperature-sensitive channels in planar lipid bilayers
- Authors
Zakharian, E.; Reusch, R.N.
- Abstract
The temperature dependence of single-channel conductance and open probability for outer membrane protein A (OmpA) of Escherichia coli were examined in planar lipid bilayers. OmpA formed two interconvertible conductance states, small channels, 36–140 pS, between 15 and 37°C, and large channels, 115–373 pS, between 21 and 39°C. Increasing temperatures had strong effects on open probabilities and on the ratio of large to small channels, particularly between 22 and 34°C, which effected sharp increases in average conductance. The data infer that OmpA is a flexible temperature-sensitive protein that exists as a small pore structure at lower temperatures, but refolds into a large pore at higher temperatures.
- Subjects
PROTEINS; ESCHERICHIA coli; LIPIDS; CELL membranes
- Publication
FEBS Letters, 2003, Vol 555, Issue 2, p229
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/S0014-5793(03)01236-5