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- Title
Neutrophil elastase up-regulates human β-defensin-2 expression in human bronchial epithelial cells
- Authors
Griffin, Siobhan; Taggart, Clifford C.; Greene, Catherine M.; O’Neill, Shane; McElvaney, Noel G.
- Abstract
Human β-defensin-2 (HBD-2) gene expression is induced by tumour necrosis factor-α, interleukin-1β and lipopolysaccharide. The objective of this study was to investigate the effect of neutrophil elastase (NE), a major pro-inflammatory protease, on HBD-2 expression. HBD-2 gene expression was assessed by reverse transcription polymerase chain reaction in the human bronchial epithelial cell line 16HBE14o− and primary normal human bronchial epithelial (NHBE) cells. Optimal HBD-2 expression was induced with 100 nM NE. Using a HBD-2-luciferase reporter construct, luciferase activity increased significantly in 16HBE14o− cells following incubation with NE. An increase in HBD-2 protein expression was observed in primary NHBE cells after incubation with NE as assessed by laser scanning cytometry. In conclusion, NE up-regulates HBD-2 expression in bronchial epithelial cells.
- Subjects
NEUTROPHILS; ELASTASES
- Publication
FEBS Letters, 2003, Vol 546, Issue 2/3, p233
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/S0014-5793(03)00577-5