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- Title
Two Types of Immunoassay Based on Nile Blue Labeling Polydopamine Nanospheres.
- Authors
Li, Li; Lu, Wenbo; Liu, Chang; Wang, Ying; Dong, Jian; Qian, Weiping
- Abstract
The sandwich-type immunoassays have been developed by using electrochemical and surface-enhanced Raman scattering (SERS) techniques for the detection of carcinoembryonic antigen (CEA). Nile blue as a kind of Raman dye has been decorated on nanospheres with polydopamine resin (PDR) via -stacking interaction. The Nile blue displays the strong SERS signals as well as a characteristic electrochemical reduction peak at 0.33V (versus Ag/AgCl). The implementation of the PDR nanospheres mixing with Au nanoparticles (AuNPs/PDR) exhibits a better electrical conductivity and large SERS enhancement. The immunoassays based on Nile blue-labeled AuNPs/PDR nanospheres have been fabricated by using electrochemical and SERS techniques for the detection of CEA. The proposed immunoassay shows higher sensitivity, high selectivity, lower detection limit and long-term stability. The performances of the electrochemical immunoassay are better than that of SERS immunoassay. For the electrochemical immunoassay, the linear range occurs from 1pg/mL to 100ng/mL () with a detection limit of 0.68pg/mL and signal-to-noise ratio of 3 in the detection of CEA. The data for the analysis of human serum samples by using the electrochemical method are acceptably consistent with those obtained from the enzyme-linked immunosorbent assay (ELISA). The proposed immunoassay exhibits a promising potential for application in clinical diagnosis. Polydopamine resin nanospheres have been synthesized to develop a novel sandwich-type immunoassay for the detection of carcinoembryonic antigen developed using electrochemical and surface enhanced Raman scattering techniques. The sandwich structure is composed of the anti-CEA/NBA/AuNPs/PDR, CEA and anti-CEA/CHIT/AuNPs/GCE.
- Subjects
ELECTROCHEMISTRY; DOPAMINE; CHEMICAL reduction; SERS spectroscopy; GOLD nanoparticles; ELECTRIC conductivity
- Publication
NANO, 2017, Vol 12, Issue 8, p-1
- ISSN
1793-2920
- Publication type
Article
- DOI
10.1142/S1793292017500928