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- Title
MUCIN AS A MARKER FOR AGGRESSIVENESS OF COLON CANCER.
- Authors
Kim, Suzy L.; Goldschmid, Steve
- Abstract
The authors used animal models of colon cancer metastasis to help elucidate the relationship between mucin production and the metastatic potential of human colon cancer cells. In a Previous study (Bresalier et al., Int J Cancer 1987;39-625), the investigators injected cells from a well-differentiated human colon adenocarcinoma cell line LS174T into the cecal wall of athymic nude mice. After serial selection, a cell line LS LiMG was obtained that had increased ability to metastasize to the liver. In a separate study (Kuan et al., cancer Res 1987;47:5715), the replica plating technique of clonal selection and immunoscreening was used to select cells with a low mucin-synthesizing capacity, LM12, and a high mucin-synthesizing capacity, HM7, from LS174T. Bresalier et al. employed these cell lines for the present study. Their paper addressed three key questions: 1) What is the quantity of mucin produced by human colon cancer cells of known metastatic potential (LS174T and LSiM6)? 2) What is the metastatic ability of colon cancer cells that differ in their ability to produce mucin (LS174T, LM12 and HM7)? 3) What is the effect of inhibiting mucin glycosylation on the ability of the tumor cells to mestastasize to the liver? In answering the first question, the authors showed that the highly metastatic cell line LSLiM6 had increased mucin production, compared with the parent cell line LS174T. The parent cell line LS174T, when injected into the cecum of 30 mice, resulted in only two mice with liver metastases. The metastatic cell line LSLiM6 produced liver metastases in 11 of 15 mice. After intrasplenic tumor cell injection, LSLiM6 colonized the liver to a greater extent and produced more tumor burden in the liver than LS174T. The difference in liver colonization was not the result of differences in the number of tumor cells initially reaching the liver, since labeling studies showed that the tumor cells from each cell line reached the liver in similar numbers. The amount...
- Subjects
MUCINS; COLON cancer; METASTASIS; CANCER cells; ANIMAL models of cancer
- Publication
American Journal of Gastroenterology (Springer Nature), 1993, Vol 88, Issue 1, p147
- ISSN
0002-9270
- Publication type
Article