We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Bioassay-led isolation ofMyrothecium verrucariaand verrucarin A as germination inhibitors ofOrobanche crenata.
- Authors
El-Kassas, R.; El-Din, Z. Karam; Beale, M. H.; Ward, J. L.; Strange, R. N.
- Abstract
El-KassasR, KaramEl-DinZ, BealeMH, WardJL&StrangeRN (2005) Bioassay-led isolation ofMyrothecium verrucariaand verrucarin A as germination inhibitors ofOrobanche crenata.Weed Research45, 212–219.A total of 188 fungal isolates was obtained from the rhizosphere ofVicia fabagrown in an Egyptian soil heavily infested withOrobanchespecies. Agar cultures of 58 isolates inhibited the germination of conditioned seed ofOrobanche crenataexposed to the germination stimulant, GR24. Filtrates of inhibitory fungi grown in liquid medium for 9–15 days were also assayed and those of five isolates, which were morphologically similar, inhibited germination even when diluted 16-fold. The fungus was identified asMyrothecium verrucaria(Alb.&Schwein.) Ditmar by its morphology and the nucleotide sequence of the ITS1 and ITS2 regions of the ribosomal repeat unit. Purification of the inhibitor to homogeneity was accomplished by solvent partitioning, flash chromatography on silica gel, semi-preparative HPLC on a reversed phase C18 column, solid phase extraction and tlc on silica gel. The inhibitor was identified as verrucarin A by nuclear magnetic resonance spectroscopy and comparison of the spectra with those of an authentic sample of the compound. A preliminary experiment demonstrated that infection ofV. fababyO. crenatacould be prevented by addition of spores of the fungus to soil infested by the parasite.
- Subjects
MYROTHECIUM verrucaria; BROOMRAPES; FAVA bean; PLANTS; BIOLOGICAL weed control; SOIL management; GERMINATION; BOTANY
- Publication
Weed Research, 2005, Vol 45, Issue 3, p212
- ISSN
0043-1737
- Publication type
Article
- DOI
10.1111/j.1365-3180.2005.00448.x