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- Title
Insights into the Cellular Localization and Functional Properties of TSPYL5 Protein.
- Authors
Silonov, Sergey A.; Smirnov, Eugene Y.; Shmidt, Eva A.; Kuznetsova, Irina M.; Turoverov, Konstantin K.; Fonin, Alexander V.
- Abstract
In recent years, the role of liquid–liquid phase separation (LLPS) and intrinsically disordered proteins (IDPs) in cellular molecular processes has received increasing attention from researchers. One such intrinsically disordered protein is TSPYL5, considered both as a marker and a potential therapeutic target for various oncological diseases. However, the role of TSPYL5 in intracellular processes remains unknown, and there is no clarity even in its intracellular localization. In this study, we characterized the intracellular localization and exchange dynamics with intracellular contents of TSPYL5 and its parts, utilizing TSPYL5 fusion proteins with EGFP. Our findings reveal that TSPYL5 can be localized in both the cytoplasm and nucleoplasm, including the nucleolus. The nuclear (nucleolar) localization of TSPYL5 is mediated by the nuclear/nucleolar localization sequences (NLS/NoLS) identified in the N-terminal intrinsically disordered region (4–27 aa), while its cytoplasmic localization is regulated by the ordered NAP-like domain (198–382 aa). Furthermore, our results underscore the significant role of the TSPYL5 N-terminal disordered region (1–198 aa) in the exchange dynamics with the nucleoplasm and its potential ability for phase separation. Bioinformatics analysis of the TSPYL5 interactome indicates its potential function as a histone and ribosomal protein chaperone. Taken together, these findings suggest a significant contribution of liquid–liquid phase separation to the processes involving TSPYL5, providing new insights into the role of this protein in the cell's molecular life.
- Subjects
CHIMERIC proteins; PHASE separation; PROTEINS; MOLECULAR chaperones; NUCLEOPLASM; RESEARCH personnel; RIBOSOMAL proteins; RIBOSOMAL DNA
- Publication
International Journal of Molecular Sciences, 2024, Vol 25, Issue 1, p39
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms25010039