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- Title
Comprehensive Analysis of hsa-miR-654-5p's Tumor-Suppressing Functions.
- Authors
Liu, Chuanyang; Min, Lu; Kuang, Jingyu; Zhu, Chushu; Qiu, Xinyuan; Wu, Xiaomin; Zhang, Tianyi; Xie, Sisi; Zhu, Lingyun
- Abstract
The pivotal roles of miRNAs in carcinogenesis, metastasis, and prognosis have been demonstrated recently in various cancers. This study intended to investigate the specific roles of hsa-miR-654-5p in lung cancer, which is, in general, rarely discussed. A series of closed-loop bioinformatic functional analyses were integrated with in vitro experimental validation to explore the overall biological functions and pan-cancer regulation pattern of miR-654-5p. We found that miR-654-5p abundance was significantly elevated in LUAD tissues and correlated with patients' survival. A total of 275 potential targets of miR-654-5p were then identified and the miR-654-5p-RNF8 regulation axis was validated in vitro as a proof of concept. Furthermore, we revealed the tumor-suppressing roles of miR-654-5p and demonstrated that miR-654-5p inhibited the lung cancer cell epithelial-mesenchymal transition (EMT) process, cell proliferation, and migration using target-based, abundance-based, and ssGSEA-based bioinformatic methods and in vitro validation. Following the construction of a protein–protein interaction network, 11 highly interconnected hub genes were identified and a five-genes risk scoring model was developed to assess their potential prognostic ability. Our study does not only provide a basic miRNA-mRNA-phenotypes reference map for understanding the function of miR-654-5p in different cancers but also reveals the tumor-suppressing roles and prognostic values of miR-654-5p.
- Subjects
EPITHELIAL-mesenchymal transition; PROGNOSIS; DISEASE risk factors; PROTEIN-protein interactions; LUNG cancer
- Publication
International Journal of Molecular Sciences, 2022, Vol 23, Issue 12, p6411
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms23126411