We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Synthesis of MIL-Modified Fe 3 O 4 Magnetic Nanoparticles for Enhancing Uptake and Efficiency of Temozolomide in Glioblastoma Treatment.
- Authors
Pulvirenti, Luca; Monforte, Francesca; Lo Presti, Francesca; Li Volti, Giovanni; Carota, Giuseppe; Sinatra, Fulvia; Bongiorno, Corrado; Mannino, Giovanni; Cambria, Maria Teresa; Condorelli, Guglielmo Guido
- Abstract
A nanometric hybrid system consisting of a Fe3O4 magnetic nanoparticles modified through the growth of Fe-based Metal-organic frameworks of the MIL (Materials Institute Lavoiser) was developed. The obtained system retains both the nanometer dimensions and the magnetic properties of the Fe3O4 nanoparticles and possesses increased the loading capability due to the highly porous Fe-MIL. It was tested to load, carry and release temozolomide (TMZ) for the treatment of glioblastoma multiforme one of the most aggressive and deadly human cancers. The chemical characterization of the hybrid system was performed through various complementary techniques: X-ray-diffraction, thermogravimetric analysis, FT-IR and X-ray photoelectron spectroscopies. The nanomaterial showed low toxicity and an increased adsorption capacity compared to bare Fe3O4 magnetic nanoparticles (MNPs). It can load about 12 mg/g of TMZ and carry the drug into A172 cells without degradation. Our experimental data confirm that, after 48 h of treatment, the TMZ-loaded hybrid nanoparticles (15 and 20 μg/mL) suppressed human glioblastoma cell viability much more effectively than the free drug. Finally, we found that the internalization of the MIL-modified system is more evident than bare MNPs at all the used concentrations both in the cytoplasm and in the nucleus suggesting that it can be capable of overcoming the blood-brain barrier and targeting brain tumors. In conclusion, these results indicate that this combined nanoparticle represents a highly promising drug delivery system for TMZ targeting into cancer cells.
- Subjects
IRON oxide nanoparticles; MAGNETIC nanoparticle hyperthermia; MAGNETIC nanoparticles; TEMOZOLOMIDE; METHYLGUANINE; X-ray photoelectron spectroscopy; BRAIN tumors
- Publication
International Journal of Molecular Sciences, 2022, Vol 23, Issue 5, p2874
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms23052874