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- Title
Function and Regulation of the Pyruvate Transporter CstA in Escherichia coli †.
- Authors
Gasperotti, Ana; Göing, Stephanie; Fajardo-Ruiz, Elena; Forné, Ignasi; Jung, Kirsten
- Abstract
Pyruvate is a central metabolite that connects many metabolic pathways in living organisms. To meet the cellular pyruvate requirements, the enterobacterium Escherichia coli has at least three pyruvate uptake systems—the H+/pyruvate symporter BtsT, and two thus far less well-characterized transporters, YhjX and CstA. BtsT and CstA belong to the putative carbon starvation (CstA) family (transporter classification TC# 2.A.114). We have created an E. coli mutant that cannot grow on pyruvate as the sole carbon source and used it to characterize CstA as a pyruvate transporter. Transport studies in intact cells confirmed that CstA is a highly specific pyruvate transporter with moderate affinity and is energized by a proton gradient. When cells of a reporter strain were cultured in complex medium, cstA expression was maximal only in stationary phase. A DNA affinity-capture assay combined with mass spectrometry and an in-vivo reporter assay identified Fis as a repressor of cstA expression, in addition to the known activator cAMP-CRP. The functional characterization and regulation of this second pyruvate uptake system provides valuable information for understanding the complexity of pyruvate sensing and uptake in E. coli.
- Subjects
ESCHERICHIA coli; MASS spectrometry; PYRUVATES; CATABOLITE repression; CLASSIFICATION; STARVATION
- Publication
International Journal of Molecular Sciences, 2020, Vol 21, Issue 23, p9068
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms21239068