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- Title
A Circulating MicroRNA Profile in a Laser-Induced Mouse Model of Choroidal Neovascularization.
- Authors
Kiel, Christina; Berber, Patricia; Karlstetter, Marcus; Aslanidis, Alexander; Strunz, Tobias; Langmann, Thomas; Grassmann, Felix; Weber, Bernhard H.F.
- Abstract
Choroidal neovascularization (CNV) is a pathological process in which aberrant blood vessels invade the subretinal space of the mammalian eye. It is a characteristic feature of the prevalent neovascular age-related macular degeneration (nAMD). Circulating microRNAs (cmiRNAs) are regarded as potentially valuable biomarkers for various age-related diseases, including nAMD. Here, we investigated cmiRNA expression in an established laser-induced CNV mouse model. Upon CNV induction in C57Bl/6 mice, blood-derived cmiRNAs were initially determined globally by RNA next generation sequencing, and the most strongly dysregulated cmiRNAs were independently replicated by quantitative reverse transcription PCR (RT-qPCR) in blood, retinal, and retinal pigment epithelium (RPE)/choroidal tissue. Our findings suggest that two miRNAs, mmu-mir-486a-5p and mmur-mir-92a-3p, are consistently dysregulated during CNV formation. Furthermore, in functional in vitro assays, a significant impact of mmu-mir-486a-5p and mmu-mir-92a-3p on murine microglial cell viability was observed, while mmu-mir-92a-3p also showed an impact on microglial mobility. Taken together, we report a robust dysregulation of two miRNAs in blood and RPE/choroid after laser-induced initiation of CNV lesions in mice, highlighting their potential role in pathology and eventual therapy of CNV-associated complications.
- Subjects
NEOVASCULARIZATION; MICRORNA; RHODOPSIN; RETINAL degeneration; MICE
- Publication
International Journal of Molecular Sciences, 2020, Vol 21, Issue 8, p2689
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms21082689