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- Title
Expression of Chemerin and Its Receptors in the Porcine Hypothalamus and Plasma Chemerin Levels during the Oestrous Cycle and Early Pregnancy.
- Authors
Smolinska, Nina; Kiezun, Marta; Dobrzyn, Kamil; Rytelewska, Edyta; Kisielewska, Katarzyna; Gudelska, Marlena; Zaobidna, Ewa; Bogus-Nowakowska, Krystyna; Wyrebek, Joanna; Bors, Kinga; Kopij, Grzegorz; Kaminska, Barbara; Kaminski, Tadeusz
- Abstract
Chemerin (CHEM) may act as an important link integrating energy homeostasis and reproductive functions of females, and its actions are mediated by three receptors: chemokine-like receptor 1 (CMKLR1), G protein-coupled receptor 1 (GPR1), and C-C motif chemokine receptor-like 2 (CCRL2). The aim of the current study was to compare the expression of CHEM and its receptor (CHEM system) mRNAs (quantitative real-time PCR) and proteins (Western blotting and fluorescent immunohistochemistry) in the selected areas of the porcine hypothalamus responsible for gonadotropin-releasing hormone production and secretion: the mediobasal hypothalamus, preoptic area and stalk median eminence during the oestrous cycle and early pregnancy. Moreover, plasma CHEM concentrations were determined using ELISA. The expression of CHEM system has been demonstrated in the porcine hypothalamus throughout the luteal phase and follicular phase of the oestrous cycle, and during early pregnancy from days 10 to 28. Plasma CHEM levels and concentrations of transcripts and proteins of CHEM system components in the hypothalamus fluctuated throughout pregnancy and the oestrous cycle. Our study was the first experiment to demonstrate the presence of CHEM system mRNAs and proteins in the porcine hypothalamus and the correlations between the expression levels and physiological hormonal milieu related to the oestrous cycle and early pregnancy.
- Subjects
ESTRUS; CHEMERIN; PREGNANCY in animals; HYPOTHALAMUS; PREOPTIC area; G protein coupled receptors; LUTEAL phase
- Publication
International Journal of Molecular Sciences, 2019, Vol 20, Issue 16, p3887
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms20163887