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- Title
Adipokinome Signatures in Obese Mouse Models Reflect Adipose Tissue Health and Are Associated with Serum Lipid Composition.
- Authors
Knebel, Birgit; Fahlbusch, Pia; Poschmann, Gereon; Dille, Matthias; Wahlers, Natalie; Stühler, Kai; Hartwig, Sonja; Lehr, Stefan; Schiller, Martina; Jacob, Sylvia; Kettel, Ulrike; Müller-Wieland, Dirk; Kotzka, Jörg
- Abstract
Adipocyte and hepatic lipid metabolism govern whole-body metabolic homeostasis, whereas a disbalance of de novo lipogenesis (DNL) in fat and liver might lead to obesity, with severe co-morbidities. Nevertheless, some obese people are metabolically healthy, but the "protective" mechanisms are not yet known in detail. Especially, the adipocyte-derived molecular mediators that indicate adipose functionality are poorly understood. We studied transgenic mice (alb-SREBP-1c) with a "healthy" obese phenotype, and obob mice with hyperphagia-induced "sick" obesity to analyze the impact of the tissue-specific DNL on the secreted proteins, i.e., the adipokinome, of the primary adipose cells by label-free proteomics. Compared to the control mice, adipose DNL is reduced in both obese mouse models. In contrast, the hepatic DNL is reduced in obob but elevated in alb-SREBP-1c mice. To investigate the relationship between lipid metabolism and adipokinomes, we formulated the "liver-to-adipose-tissue DNL" ratio. Knowledge-based analyses of these results revealed adipocyte functionality with proteins, which was involved in tissue remodeling or metabolism in the alb-SREBP-1c mice and in the control mice, but mainly in fibrosis in the obob mice. The adipokinome in "healthy" obesity is similar to that in a normal condition, but it differs from that in "sick" obesity, whereas the serum lipid patterns reflect the "liver-to-adipose-tissue DNL" ratio and are associated with the adipokinome signature.
- Subjects
LIPID metabolism; OBESITY; PHENOTYPES; PROTEOMICS; FAT cells
- Publication
International Journal of Molecular Sciences, 2019, Vol 20, Issue 10, p2559
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms20102559