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- Title
Characterization of Copy Number Variation’s Potential Role in Marek’s Disease.
- Authors
Lingyang Xu; Yanghua He; Yi Ding; Guirong Sun; Carrillo, Jose Adrian; Yaokun Li; Ghaly, Mona M.; Li Ma; Huanmin Zhang; Liu, George E.; Jiuzhou Song
- Abstract
Marek’s Disease (MD) is a highly contagious pathogenic and oncogenic disease primarily affecting chickens. Chicken Lines 63 and 72, as well as their recombinant congenic strains (RCS) with varied susceptibility to MD, are ideal models to study the complex mechanisms of genetic resistance to MD. In this study, we investigated copy number variation (CNV) in these inbred chicken lines using the Affymetrix Axiom HD 600 K SNP genotyping array. We detected 393 CNV segments across all ten chicken lines, of which 12 CNVs were specifically identified in Line 72. We then assessed genetic structure based on CNV and observed markedly different patterns. Finally, we validated two deletion events in Line 72 and correlated them with genes expression using qPCR and RNA-seq, respectively. Our combined results indicated that these two CNV deletions were likely to contribute to MD susceptibility.
- Subjects
MAREK'S disease; CHICKEN diseases; RECOMBINANT antibodies; GENOTYPES; RNA sequencing; POLYMERASE chain reaction
- Publication
International Journal of Molecular Sciences, 2017, Vol 18, Issue 5, p1020
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms18051020