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- Title
过表达AGPase编码基因对甘蓝型油菜种子淀粉含量及 油脂合成的影响.
- Authors
赵凯琴; 杨清辉; 张云云; 罗延青; 王敬乔; 李劲峰; 陈苇; 田正书
- Abstract
In order to understand the function of adenosine diphosphate glucose pyrophosphorylase (AGPase) gene and its relationship with the changes in oil content of rapeseeds, two types of specific promoter-gene expression vectors were constructed: pMB-DOF-Glgc (overexpress the exogenous AGPase gene Glgc in early embryos) and pMB-Cruc-Glgc (overexpress the exogenous Glgc in late embryos). By detection of transgenic positive plants subsequently, it was intended to increase starch synthesis in embryo, promote synthesis of fatty acids, and achieve the purpose of increasing the oil content of rape seeds. Ten single-copy positive strains were selected from the transformed positive plants in each vector group by qPCR method. The relative starch content in seeds at different developmental stages and oil content of mature seeds of the above positive plants were analyzed. Oil content analysis was performed on mature seeds of all transformed positive plants. Results presented that the average relative content of the starch in single-copy plant seeds in DOF: Glgc group and Cruc: Glgc group increased from the pre-development stage (22 DAF) and the middle stage development (35 DAF), respectively. The peaks of starch accumulation in two mock-vehicle controls were 9.43% and 9.76%, at 31 DAF respectively. Maximum starch content of the DOF: Glgc group also appeared at 31 DAF, reaching 12.33%. While in Cruc: Glgc group it appeared at 35 DAF, reaching 12.92%. The average oil content of mature seeds in the two control groups was 40.12% and 40.00%, and the aver⁃ age oil content of mature seeds in the DOF: Glgc group and Cruc: Glgc group were 40.35% and 40.26%, respective⁃ ly. For 150 transgenic lines, oil content of two Cruc: Glgc group were 40.58% and 40.60% respectively,while the average oil content of the controls were 40.30% and 40.33%. The analysis results revealed that the starch content of DOF: Glgc and Cruc: Glgc transformants significantly increased, and the peak value of the increase was consistent with the promoter's spatiotemporal specificity. However, the increase in oil content of the transformant seeds was not significant. Thus suggested that increasing the starch content in rape embryos might not change the final carbon flow distribution and thus affect the seed oil content.
- Subjects
ADENOSINE diphosphate; OILSEEDS; TRANSGENIC plants; STARCH; OIL changes; RAPESEED oil
- Publication
Chinese Journal of Oil Crop Sciences, 2021, Vol 43, Issue 3, p426
- ISSN
1007-9084
- Publication type
Article
- DOI
10.19802/j.issn.1007-9084.2020016